The Impact of the Functional Layer Composition of Glucose Test-Strips on the Stability of Electrochemical Response

Herein, the impact of chemical stability RedOx mediator ferricyanide, K3[Fe(CN)6] (FC), a type buffer solution used for bioreceptor preparation, gel composition (carboxymethylcellulose, CMC, Aerosile, AS, and alginate, ALG) on long term glucose test-strips their analytical performance was examined. By simple addition ALG to functional aiming improve its viscosity, we managed enhance sensitivity conventional CMC-containing amperometric from 3.3 µA/mM 3.9 extend shelf life 8 months 1.7 years. Moreover, during course investigations, it revealed that activity enzyme in dependence with did not linearly correlate dried layer, entire long-term electrochemical signal determined by FC stability. The most stable sensitive were obtained screen-printing approach containing 24 mg/mL GOx prepared citrate pH 6, 200 10 CMC supplemented 25 ALG.